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KMID : 0545120040140020363
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 2 p.363 ~ p.366
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Breeding of L(+)-Lactic Acid Producing Strain by Low-Energy Ion Implantation
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GE, CHUN-MEI
GU, SHAO-BIN/ZHOU, XIU-HONG/YAO, JIAN-MING/PAN, REN-RUI/YU, ZENG-LIANG
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Abstract
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In order to obtain an industrial strain with higher L(+)-lactic acid yield, the wild type strain Rhizopus oryzue PW352 was mutated by means of Nitrogen ions implantation (15 Kev, 7.8×10^(14) -2.08×10^(15) ions/㎠) and two mutants RE3303 and RF9052 were isolated. After 36 h shake-flask cultivation, the concentration of L(+)-lactic acid reached 131- 136 g/l, the conversion rate of glucose was as high as 86%- 90% and the productivity was 3.61 g/I·h. It was almost a 75% increase in lactic acid production compared with the wild type strain. Maximum fermentation temperature of RF9052 was increased to 45℃ from original 36℃. At the same time, the preferred range of fermentation temperature of RF9052 was broadened compared with PW352.
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